C4Investigator is a novel bioinformatics pipeline for comprehensive, high-throughput characterization of human C4 sequence from short-read sequencing data meant to investigate C4 variation. Using paired-end targeted or whole genome sequence data as input, C4Investigator determines gene copy number for overall C4, C4A, C4B, C4(Rodger), C4(Ch), C4(L), and C4(S), additionally, C4Ivestigator reports the full overall C4 aligned sequence, enabling nucleotide level analysis of C4.

C4Investigator can be accessed in our GitHub page here.